gai (Addgene inc)
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93
Structured Review
Addgene inc
gai
Gai, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pslq2816+ppb/pSLQ2816+pPB%3A+CAG-PYL1-VPR-p2A-GID1-ABI-WPRE+PGK-GAI-tagBFP-SpdCas9+(Plasmid+%2384261)/pm37223115-162-30-36
Average 93 stars, based on 2 article reviews
Gai, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pslq2816+ppb/pSLQ2816+pPB%3A+CAG-PYL1-VPR-p2A-GID1-ABI-WPRE+PGK-GAI-tagBFP-SpdCas9+(Plasmid+%2384261)/pm37223115-162-30-36
Average 93 stars, based on 2 article reviews
gai - by Bioz Stars,
2026-10
93/100 stars
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Polymerase Chain Reaction:Article Title: Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor. Article Snippet: 1Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana 1000, Slovenia; 2EN-FIST Centre of Excellence, Trg Osvobodilne fronte 13, Ljubljana 1000, Slovenia; 3Department of Hematology, Division of Internal Medicine, University Medical Center Ljubljana, Zalo ska 7, Ljubljana 1000, Slovenia; 4Graduate School of Biomedicine, University of Ljubljana, Ljubljana 1000, Slovenia Article Title: Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor Article Snippet: Truncated NFAT2 was cloned into pcDNA3 (Invitrogen) and connected at N-terminus via a 10 amino acid GS linker with domain ABI (PCR amplified from pSLQ2816 pPB [Addgene plasmid no. 84261]), GAI (PCR amplified from pSLQ2816 pPB [Addgene plasmid no. 84261]) or with DmrA (PCR amplified from pHet-NucI [iDimerize, Takara Bio USA]). .. The PYL1 and GID1 domains were PCR amplified from Amplification:Article Title: Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor. Article Snippet: 1Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana 1000, Slovenia; 2EN-FIST Centre of Excellence, Trg Osvobodilne fronte 13, Ljubljana 1000, Slovenia; 3Department of Hematology, Division of Internal Medicine, University Medical Center Ljubljana, Zalo ska 7, Ljubljana 1000, Slovenia; 4Graduate School of Biomedicine, University of Ljubljana, Ljubljana 1000, Slovenia Article Title: Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor Article Snippet: Truncated NFAT2 was cloned into pcDNA3 (Invitrogen) and connected at N-terminus via a 10 amino acid GS linker with domain ABI (PCR amplified from pSLQ2816 pPB [Addgene plasmid no. 84261]), GAI (PCR amplified from pSLQ2816 pPB [Addgene plasmid no. 84261]) or with DmrA (PCR amplified from pHet-NucI [iDimerize, Takara Bio USA]). .. The PYL1 and GID1 domains were PCR amplified from Plasmid Preparation:Article Title: Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor. Article Snippet: 1Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana 1000, Slovenia; 2EN-FIST Centre of Excellence, Trg Osvobodilne fronte 13, Ljubljana 1000, Slovenia; 3Department of Hematology, Division of Internal Medicine, University Medical Center Ljubljana, Zalo ska 7, Ljubljana 1000, Slovenia; 4Graduate School of Biomedicine, University of Ljubljana, Ljubljana 1000, Slovenia Article Title: Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor Article Snippet: Truncated NFAT2 was cloned into pcDNA3 (Invitrogen) and connected at N-terminus via a 10 amino acid GS linker with domain ABI (PCR amplified from pSLQ2816 pPB [Addgene plasmid no. 84261]), GAI (PCR amplified from pSLQ2816 pPB [Addgene plasmid no. 84261]) or with DmrA (PCR amplified from pHet-NucI [iDimerize, Takara Bio USA]). .. The PYL1 and GID1 domains were PCR amplified from |